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By Winifred M. Watkins (auth.), Harry Harris, Kurt Hirschhorn (eds.)

ISBN-10: 1461582881

ISBN-13: 9781461582885

ISBN-10: 1461582903

ISBN-13: 9781461582908

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The presence of this sequence was recently demonstrated in 1 blood group active poly(glycosyl) ceramides (Zdebska and Koscielak, 1978) and a second anti-l serum (Woj) was shown to be specific for this structure. , 1978). 3GlcNAc(l~3)Gal was inactive. Kabat et al. (1978) further delineated the relationship between the structure recognized by the group 1 anti-I sera and by anti-Type XIV pneumococcal serum. 3GlcNAc(l~3)Gal and both were only marginally more active than 13Gal(l~4)GlcNAc. The combining site of the Type XIV antiserum is therefore directed mainly towards the terminal disaccharide unit of the Type 2 precursor chain.

1958). , 1968) have also been used as acceptor substrates for the detection of the a-2-L-fucosyltransferase. In general, when radioactive GDP-L-fucose is used as the sugar donor, determination of the positional linkage of the added L-(I4C]fucose is more difficult to ascertain with macromolecular substrates than when low-molecular-weight acceptors are employed. , 1976). It is therefore very important to use methods that distinguish the product of action of the a-2-L-fucosyltransferase from the products given by the other fucosyltransferases.

The nature of the difference between the subgroups is still controversial. , 1969). , 1977; Kisailus and Kabat, 1978). , 1967; Williams and Voak, 1972) have established unequivocally that there are far fewer A sites on A2 cells than on AI cells. More recent work has indicated that in both AI and A2 phenotypes there are several different populations of red cells varying from antigen-rich to antigen-poor cells. Using a fluorescent anti-AI reagent, Rochant et al. (1976) observed that AI cells were a mixture of at least three populations: 20-30% of the cells gave strong fluorescence, 50-60% of the cells gave intermediate fluorescence and the remainder gave only very pale fluorescence.

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Advances in Human Genetics 10 by Winifred M. Watkins (auth.), Harry Harris, Kurt Hirschhorn (eds.)

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